P62-mediated mitophagy inducer

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

P62 介导的线粒体自噬诱导剂 (PMI) 是一种小分子 p62 介导的线粒体自噬诱导剂，可在不招募 Parkin 或破坏膜电位的情况下激活线粒体自噬，并在缺乏功能齐全的 PINK1/Parkin 通路的细胞中保留活性； 1 uM 诱导 NQO1 活性加倍，并上调 Nrf2 和 ARE 依赖性抗氧化基因；增加自噬衔接分子 P62/SQSTM1 的表达和信号传导，迫使线粒体进入自噬；破坏 Nrf2-Keap1 相互作用。

P62-mediated mitophagy inducer (PMI) is a small molecule p62-mediated mitophagy inducer that activates mitophagy without recruiting Parkin or collapsing membrane potential and retains activity in cells devoid of a fully functional PINK1/Parkin pathway; induces doubling NQO1 activity at 1 uM, and upregulates Nrf2 and ARE-dependent antioxidant genes; increases the expression and signaling of the autophagic adaptor molecule P62/SQSTM1 and forces mitochondria into autophagy; disrupts the Nrf2-Keap1 interaction.

P62-mediated mitophagy inducer (10 μM; 0, 1, 3, 6, 24 h) stabilizes Nrf2 and (10 μM; 9 h) upregulates P62 expression activating mitophagy.P62-mediated mitophagy (10 μM; 24 h) acts downstream of the PINK1/Parkin signaling pathway in MEFs.P62-mediated mitophagy inducer positively affects mitochondrial poly-ubiquitination and coupling. RT-PCR Cell Line:MEFs Concentration:10 μM Incubation Time:9 h Result:Significantly increased p62 mRNA levels.Immunofluorescence Cell Line:MEFs Concentration:10 μM Incubation Time:24 h Result:Demonstrated Parkin-independent induction of mitochondrial recruitment of P62.Western Blot Analysis Cell Line:MEFs Concentration:10 μM Incubation Time:0, 1, 3, 6, 24 h Result:Exhibited maximum Nrf2 levels after 6 h and remained elevated at 24 h.

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PMI

Autophagy

Mitophagy

Mitophagy

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1809031-84-2

392.156

C14H9IN4O2

>98% (HPLC)

In Vitro:?DMSO : 8.33 mg/mL (21.24 mM)

C1=CC(=CC(=C1)[N+](=O)[O-])C2=CN(N=N2)C3=CC(=CC=C3)I

1-(3-iodophenyl)-4-(3-nitrophenyl)-1H-1,2,3-triazole

(-20℃)

With Ice Pack

≥ 2 years